bovine oligo-microarray slide (embryogene embv3 Search Results


bovine oligo-microarray slide (embryogene embv3  (Agilent technologies)
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Agilent technologies bovine oligo-microarray slide (embryogene embv3
Quantification (log 2 of fold-change; mean ± SEM) of the mRNA profiles in granulosa cells after a Long (7-day) compared to conventional (4-day, reference group) FSH treatment. Cells were obtained from the FSH-stimulated follicles 24 h after exogenous LH treatment in both groups. Bars extending to the right and left of zero represent up- and down-regulated genes, respectively, in the Long FSH group. RT-PCR data analysis was performed using REST 2009 program. Light grey bars represent expression of transcript in <t>microarray</t> experiment while dark grey bars represent expression of the same transcript obtained by RT-PCR. Asterisk (*) by the side of a bar represent statistical difference ( P < 0.1) between long FSH in relation to the conventional FSH in RT-PCR experiments
Bovine Oligo Microarray Slide (Embryogene Embv3, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quantification (log 2 of fold-change; mean ± SEM) of the mRNA profiles in granulosa cells after a Long (7-day) compared to conventional (4-day, reference group) FSH treatment. Cells were obtained from the FSH-stimulated follicles 24 h after exogenous LH treatment in both groups. Bars extending to the right and left of zero represent up- and down-regulated genes, respectively, in the Long FSH group. RT-PCR data analysis was performed using REST 2009 program. Light grey bars represent expression of transcript in microarray experiment while dark grey bars represent expression of the same transcript obtained by RT-PCR. Asterisk (*) by the side of a bar represent statistical difference ( P < 0.1) between long FSH in relation to the conventional FSH in RT-PCR experiments

Journal: BMC Genomics

Article Title: Transcriptome analysis of granulosa cells after conventional vs long FSH-induced superstimulation in cattle

doi: 10.1186/s12864-018-4642-9

Figure Lengend Snippet: Quantification (log 2 of fold-change; mean ± SEM) of the mRNA profiles in granulosa cells after a Long (7-day) compared to conventional (4-day, reference group) FSH treatment. Cells were obtained from the FSH-stimulated follicles 24 h after exogenous LH treatment in both groups. Bars extending to the right and left of zero represent up- and down-regulated genes, respectively, in the Long FSH group. RT-PCR data analysis was performed using REST 2009 program. Light grey bars represent expression of transcript in microarray experiment while dark grey bars represent expression of the same transcript obtained by RT-PCR. Asterisk (*) by the side of a bar represent statistical difference ( P < 0.1) between long FSH in relation to the conventional FSH in RT-PCR experiments

Article Snippet: Overall, 6 hybridizations were performed using a custom-built bovine oligo-microarray slide (EmbryoGENE EMBV3 manufactured by Agilent; Design ID: 028298, GEO accession # GPL13226).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Microarray